|
KMID : 0380219940270010060
|
|
Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 1 p.60 ~ p.63
|
|
|
Effect of Amino Acid Substiution on the Subtype Specificity of preS2 Epitope in Hepatitis B Surface Antigen
|
|
|
|
|
Abstract
|
|
|
Abstract:
@EN Antigenicity of the preS2 region of hepatitis B surface antigen was characterized by site-directed mutagenesis. Studies of serial deletions located the antigenic epitope between amino acid residues 132and 143. Differences in the amino acid
sequence
between the adr and adω2 subtypes occur at residues 132 and 141. Amino acid substitutions at residue 132 in the adr sequence did not affect antigenicity of the preS2 to the monoclonal antibody H8, which is specific to the adr subtype preS2
sequence.
However, substitutions at residue 141 did lead to a loss of antigenicity. A change of amino acid residue 141 from leucine to phenylalanine in the preS2 sequence of the adω2 subtype was sufficient for the sequence to be recognized by the
monoclonal
antibody H8. These results indicate that a single amino acid, Phe141, in the preS2 epitope is responsible for determining the subtype specificity recognized by the monoclonal antibody H8.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|